IWP-4

IWP-4 is an inhibitor of Wnt production that impairs Wnt pathway activity in vitro with an IC50 value of 25 nM. IWP-4 inactivates Porcupine, a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. At 5 µM, IWP-4 has been shown to block Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 receptor and the scaffold protein Dishevelled, preventing the accumulation of beta-catenin. This compound has been used to induce cardiomyocyte differentiation from human pluripotent stem cells.Formal Name: N-(6-methyl-2-benzothiazolyl)-2-[[3,4,6,7-tetrahydro-3-(2-methoxyphenyl)-4-oxothieno[3,2-d]pyrimidin-2-yl]thio]-acetamide. CAS Number: 686772-17-8. Synonyms: Inhibitor of Wnt Production-4. Molecular Formula: C23H20N4O3S3. Formula Weight: 496.6. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMF:PBS(pH7.2) (1:2): 0.3 mg/ml, DMSO: 2 mg/ml. lambdamax: 279, 302, 343 nm. SMILES: O=C(CSC(N1C2=CC=CC=C2OC)=NC3=C(SCC3)C1=O)NC4=NC5=C(C=C(C)C=C5)S4. InChi Code: InChI=1S/C23H20N4O3S3/c1-13-7-8-14-18(11-13)33-22(24-14)26-19(28)12-32-23-25-15-9-10-31-20(15)21(29)27(23)16-5-3-4-6-17(16)30-2/h3-8,11H,9-10,12H2,1-2H3,(H,24,26,28). InChi Key: RHUJMHOIQBDFQR-UHFFFAOYSA-N.