Indo-1 (sodium salt)

Indo-1 is a ratiometric fluorescent calcium indicator. It is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium. Indo-1 is prone to photobleaching, which limits its usefulness in methods involving microscopy.Formal Name: 1-[2-amino-5-(6-carboxyindol-2-yl)phenoxyl-2-(2'-amino-5'-methyl-phenoxy)ethane]-N,N,N',N'-tetraacetic acid, pentasodium salt. Molecular Formula: C32H26N3O12 . 5Na. Formula Weight: 759.5. Purity: >90%. Emission: 475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound). Excitation: 349-364 nm. Formulation: (Request formulation change), A solid. Solubility: Water: Soluble. SMILES: [O-]C(CN(CC([O-])=O)C1=CC=C(C2=CC3=C(C=C(C([O-])=O)C=C3)N2)C=C1OCCOC4=C(N(CC([O-])=O)CC([O-])=O)C=CC(C)=C4)=O.[Na+].[Na+].[Na+].[Na+].[Na+]. InChi Code: InChI=1S/C32H31N3O12.5Na/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22,,,,,/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45. InChi Key: LFNRCUIINCZPOA-UHFFFAOYSA-I.