Hoechst 33258

Hoechst 33258 is a cell-permeable, benzimidazole dye that binds to the minor groove of double stranded DNA with preference for adenine and thymine-rich sequences. It emits blue fluorescence (excitation 352 nm/emission maximum 461 nm) when bound to DNA in either live or fixed cells and is useful as a marker of nuclei for cell cycle studies and to distinguish nuclear morphology in apoptotic cells.Formal Name: 4-[6-(4-methyl-1-piperazinyl)[2,6'-bi-1H-benzimidazol]-2'-yl]-phenol, trihydrochloride. CAS Number: 23491-45-4. Synonyms: Bisbenzimide, HOE 33258, NSC 322921, Pibenzimol. Molecular Formula: C25H24N6O . 3HCl. Formula Weight: 533.9. Purity: >95%. Emission: 461 nm. Excitation: 352 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 20 mg/ml, PBS (pH 7.2): 5 mg/ml. lambdamax: 236, 270, 351 nm. SMILES: CN1CCN(C2=CC(N=C(C3=CC(N=C(C4=CC=C(O)C=C4)N5)=C5C=C3)N6)=C6C=C2)CC1.Cl.Cl.Cl. InChi Code: InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16,,,/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29),3*1H. InChi Key: SMNPLAKEGAEPJD-UHFFFAOYSA-N.