GM1489

GM1489, an analog of GM 6001, acetohydroxamic acid, is a potent broad spectrum inhibitor of matrix metalloproteinases (MMPs). Also reduces the inflammatory and the hyperproliferative responses that occur following topical phorbol ester application. Nomenclature: N-[(2R)-2-(Carboxymethyl)-4-methylpentanoyl]-L-tryptophan-(S)-methylbenzylamide , Reported Ki values are as follows: , Ki = 0.2nM for interstitial collagenase (MMP-1), Ki = 500nM for gelatinase A (MMP-2), Ki = 20uM for stromelysin (MMP-3), Ki = 100nM for neutrophil collagenase (MMP-8), Ki = 100nM for gelatinase B (MMP-9)]. Applications: Suitable for use in Cell Culture. Recommended Dilutions: Cell Culture: 10-25uM (1mg/ml in DMSO = 2.57mM) , Recommended Dilution Buffer: , 50mM Tris pH7.5, 150mM NaCl, 20mM CaCl2-2H2O. Purity: > 98% by HPLC Appearance: White to off-white powder, Melting Point: 181-182ºC, Concentration: It is recommended that the concentration of GM6001 in aqueous working solutions is determined upon dilution and confirmed prior to each use. Relative concentrations of GM6001 in aqueous solutions may be determined by measuring the absorbance of the solution (tyrosine) at 280 nm. Reconstitution: Reconstitute in DMSO (400mg/ml). Other organic solvents (ethanol) have also been used. G8237 will precipitate from aqueous solutions exceeding 100uM. Aqueous dilutions should be performed using low salt buffers to prevent precipitation from solution. Quality Control:, H-NMR Spectrum: Conforms, Mass Spectrum: 389 [M+1], Thin Layer Chromatography (ethylacetate:methanol = 6:1): Single spot, Storage and Stability: -20°C. Protect from moisture. Following reconstitution aliquot and freeze (-20°C). This product is stable for 2 years as supplied. Stock solutions are stable for up to 6 months at -20°C.