Fluorescein 5-isothiocyanate

Fluorescein 5-isothiocyanate (5-FITC) is an isomer of fluorescein isothiocyanate (FITC), which is commonly used as a mixture of the 5- and 6-isothiocyanate isomers. It reacts with amine and thiol groups to form conjugates with proteins, lipids, and other molecules for detection by a variety of fluorescent-based applications. 5-FITC displays excitation/emission maxima of 494/519 nm, respectively.Formal Name: 3',6'-dihydroxy-5-isothiocyanato-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one. CAS Number: 3326-32-7. Synonyms: 5-FITC, FITC isomer I, 5-Isothiocyanatofluorescein. Molecular Formula: C21H11NO5S. Formula Weight: 389.4. Purity: >90%. Emission: 519 nm. Excitation: 494 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 14 mg/ml, DMSO: 14 mg/ml, Ethanol: 10 mg/ml, PBS (pH 7.2): 0.2 mg/ml. lambdamax: 226, 278 nm. SMILES: OC1=CC=C(C2(C(C=CC(N=C=S)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1. InChi Code: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H. InChi Key: MHMNJMPURVTYEJ-UHFFFAOYSA-N.