Fluorescein 5(6)-isothiocyanate

Fluorescein 5(6)-isothiocyanate (FITC) is an amine-reactive fluorescent probe. It reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based applications. FITC displays excitation/emission maxima of 485/535 nm, respectively.Formal Name: 3',6'-dihydroxy-5(or 6)-isothiocyanato-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one. CAS Number: 27072-45-3. Synonyms: FITC, Fluorescein isothiocyanate. Molecular Formula: C21H11NO5S. Formula Weight: 389.4. Purity: >90% (mixture of isomers). Emission: 535 nm. Excitation: 485 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS (pH 7.2) (1:2): 0.33 mg/ml, DMSO: 5 mg/ml, Ethanol: slightly soluble, PBS (pH 7.2): slightly soluble. lambdamax: 224 nm. SMILES: OC1=CC=C2C(OC3=CC(O)=CC=C3C24C(C=CC(N=C=S)=C5)=C5C(O4)=O)=C1.OC6=CC=C7C(OC8=CC(O)=CC=C8C79C(C=C(N=C=S)C=C%10)=C%10C(O9)=O)=C6. InChi Code: InChI=1S/2C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21,23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h2*1-9,23-24H. InChi Key: AEWCEHLYVLRLBF-UHFFFAOYSA-N.