Fluo-4 AM

Fluo-4 acetoxymethyl ester (Fluo-4 AM) is a cell-permeable fluorescent calcium indicator. It is cleaved by intracellular esterases to release Fluo-4, which binds to calcium with a Kd value of 345 nM and displays excitation/emission maxima of 490/520 nm, respectively. Fluo-4 AM has been used to measure intracellular calcium levels in live cells by fluorescent microscopy or flow cytometry.Formal Name: N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine (acetyloxy)methyl ester. CAS Number: 273221-67-3. Synonyms: Fluo-4 Acetoxymethyl ester. Molecular Formula: C51H50F2N2O23. Formula Weight: 1096.9. Purity: >95%. Emission: 520 nm. Excitation: 490 nm. Formulation: (Request formulation change), A solid. Solubility: Methanol: soluble. SMILES: FC1=C(OCOC(C)=O)C=C(O2)C(C(C3=CC(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C(C=C3)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(C2=CC5=O)C=C5F)=C1. InChi Code: InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3. InChi Key: QOMNQGZXFYNBNG-UHFFFAOYSA-N.