EZ Cap(TM) Cy5 Firefly Luciferase mRNA (mo5U)

EZ Cap(TM) Cy5 Firefly Luciferase mRNA encodes for luciferase, a protein originally isolated from the firefly P. pyralis, that catalyzes the ATP-dependent oxidation of the molecule luciferin resulting in chemiluminescence at a wavelength of approximately 560 nm. It is capped using a co-transcriptional capping method, resulting in the naturally occurring Cap 1 structure with high capping efficiency. EZ Cap(TM) Firefly Luciferase mRNA is also polyadenylated and modified with 5-methyoxy-UTP (mo5U) to reduce host cell immune response, increase mRNA stability, and enhance translation initiation. Cy5 (Cay-30752) is a cyanine-containing fluorochrome with excitation/emission maxima of 650/670 nm, respectively. Encapsulation of EZ Cap(TM) Cy5 Firefly Luciferase mRNA (mo5U) in lipid nanoparticles (LNPs) can be used for mRNA delivery and expression of luciferase protein in vitro.Synonyms: EZ CapTM Cy5 Firefly Luciferase mRNA (5-moUTP). Emission: 670 nm. Excitation: 650 nm. Formulation: (Request formulation change), 1 mM Sodium citrate, pH 6.4.