DFHBI 1T

DFHBI 1T is a fluorescent probe for RNA. It displays excitation/emission maxima of 447/501 nm, respectively, and fluoresces upon binding to RNA aptamers, such as Broccoli, Spinach, or Spinach2. DFHBI 1T has been used to image RNA in live cells.Formal Name: (5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one. CAS Number: 1539318-36-9. Molecular Formula: C13H9F5N2O2. Formula Weight: 320.2. Purity: >98%. Emission: 501 nm. Excitation: 447 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:4): 0.20 mg/ml, Ethanol: 1 mg/ml. lambdamax: 363 nm. SMILES: FC(C=C1/C=C2N=C(C)N(CC(F)(F)F)C\2=O)=C(C(F)=C1)O. InChi Code: InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-. InChi Key: AWYCLBWNRONMQC-WMZJFQQLSA-N.