Cyano-myracrylamide

Cyano-myracrylamide (CMA) is an inhibitor of zinc finger DHHC domain-containing (zDHHC) palmitoyltransferase 20 (zDHHC20, IC50 = 1.35 µM in a cell-free assay). It selectively inhibits zDHHC20 over acyl-protein thioesterase 1 (APT-1) and APT-2 at 50 µM. CMA (5-20 µM) inhibits S-acylation of Legionella E3 ligase GobX, MyD88, and Ras, which are substrates of zDHHC20, zDHHC9, and zDHHC6, respectively, in HEK293T cells expressing recombinant Legionella GobX, recombinant human MyD88, or endogenous Ras. Unlike the zDHHC inhibitor 2-bromopalmitate (2BP), CMA is not toxic to HEK293T cells at 40 µM.Formal Name: N-(cyanomethyl)-N-tetradecyl-2-propenamide. CAS Number: 2801702-34-9. Synonyms: CMA. Molecular Formula: C19H34N2O. Formula Weight: 306.5. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml. lambdamax: 234 nm. SMILES: CCCCCCCCCCCCCCN(CC#N)C(C=C)=O. InChi Code: InChI=1S/C19H34N2O/c1-3-5-6-7-8-9-10-11-12-13-14-15-17-21(18-16-20)19(22)4-2/h4H,2-3,5-15,17-18H2,1H3. InChi Key: RIIUZCPEMOKUSJ-UHFFFAOYSA-N.