Cl-Necrostatin-1

Cl-Necrostatin-1 is an inhibitor of receptor-interacting protein kinase 1 (RIPK1). It inhibits RIPK1 activity when used at concentrations of 0.3, 3, and 30 µM. It also inhibits TNF-alpha-induced necroptosis in Jurkat cells deficient in Fas-associated death domain protein (FADD, EC50 = 180 nM), a modification that prevents caspase activation in response to death-domain receptor signaling. Cl-Necrostatin-1 (2 µl of a 4 mM solution, i.c.v.) reduces infarct size when administered pre- and post-occlusion, or post-occlusion only, in a mouse model of middle cerebral artery occlusion (MCAO).Formal Name: 5-[(7-chloro-1H-indol-3-yl)methyl]-3-methyl-2-thioxo-4-imidazolidinone. CAS Number: 862377-51-3. Synonyms: 7-Cl-Nec-1, 7-Cl-Necrostatin-1, Nec-1f, Necrostatin-1f. Molecular Formula: C13H12ClN3OS. Formula Weight: 293.8. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMF:PBS (pH 7.2) (1:1): 0.5 mg/ml, DMSO: 14 mg/ml, Ethanol: 3 mg/ml. lambdamax: 221, 267 nm. SMILES: O=C(C(N1)CC2=CNC3=C(Cl)C=CC=C32)N(C)C1=S. InChi Code: InChI=1S/C13H12ClN3OS/c1-17-12(18)10(16-13(17)19)5-7-6-15-11-8(7)3-2-4-9(11)14/h2-4,6,10,15H,5H2,1H3,(H,16,19). InChi Key: OKOAUGRWEJDVCB-UHFFFAOYSA-N.