Chymotrypsin Substrate II, Fluorogenic

Chymotrypsin substrate II, fluorogenic is a fluorogenic substrate for chymotrypsin and chymotrypsin-like proteases. Upon enzymatic cleavage by proteases, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify protease activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-(3-carboxy-1-oxopropyl)-L-alanyl-L-alanyl-L-prolyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-phenylalaninamide. CAS Number: 88467-45-2. Synonyms: Suc-AAPF-AMC, Suc-Ala-Ala-Pro-Phe-AMC, Suc-Ala2-Pro-Phe-AMC, Succinyl-Ala-Ala-Pro-Phe-AMC. Molecular Formula: C34H39N5O9. Formula Weight: 661.7. Purity: >98%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble: >10 mg/ml, Ethanol: Soluble: >10 mg/ml. SMILES: O=C([C@H](C)NC([C@H](C)NC(CCC(O)=O)=O)=O)N1CCC[C@H]1C(N[C@H](C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)CC4=CC=CC=C4)=O. InChi Code: InChI=1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-32(45)25(17-22-8-5-4-6-9-22)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,37,45)(H,38,46)(H,41,42)/t20-,21-,25-,26-/m0/s1. InChi Key: FMVUZZZUYBXQGR-KCXKOMAXSA-N.