Calcein Blue AM

Calcein Blue AM is a fluorogenic dye that is used to assess cell viability. As the acetoxymethyl ester, Calcein Blue AM is non-fluorescent but cell-permeant and is hydrolyzed to the fluorescent probe Calcein Blue (Cay-20636) by intracellular esterases. Calcein Blue is retained by living cells and displays excitation/emission maxima of 360/445 nm, respectively. Calcein Blue AM is a blue-emitting variant of the green fluorophore Calcein AM (Cay-14948), which has been used to evaluate cell viability and cytotoxicity.Formal Name: N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[(7-hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl)methyl]-glycine, (acetyloxy)methyl ester. CAS Number: 168482-84-6. Synonyms: Calcein Blue Acetoxymethyl ester. Molecular Formula: C21H23NO11. Formula Weight: 465.4. Purity: >90%. Emission: 445 nm. Excitation: 360 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: soluble. SMILES: OC1=CC=C(C(C)=CC(O2)=O)C2=C1CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O. InChi Code: InChI=1S/C21H23NO11/c1-12-6-18(26)33-21-15(12)4-5-17(25)16(21)7-22(8-19(27)31-10-29-13(2)23)9-20(28)32-11-30-14(3)24/h4-6,25H,7-11H2,1-3H3. InChi Key: GSFJGCPBBHLSPH-UHFFFAOYSA-N.