b-AP15

Proteasome-associated deubiquitinases (DUBs) release ubiquitin from proteasome-targeted ubiquitinated proteins, regenerating free ubiquitin. b-AP15 is an inhibitor of ubiquitin-specific-processing protease 14 (USP14) and ubiquitin carboxyl-terminal hydrolase isozyme L5 (UCHL5), two proteasome-associated DUBs. It inhibits DUB activity in purified 19S proteasomes with an IC50 value of 2.1 µM. It exhibits little or no activity against several other DUBs. Through its effects on USP14 and UCHL5, b-AP15 blocks tumor progression in vivo in mice and prevents organ infiltration in mouse models of myeloid leukemia. b-AP15 also renders tumor cells sensitive to TNF-mediated apoptosis by natural killer and T cells.Formal Name: 3E,5E-bis[(4-nitrophenyl)methylene]-1-(1-oxo-2-propen-1-yl)-4-piperidinone. CAS Number: 1009817-63-3. Molecular Formula: C22H17N3O6. Formula Weight: 419.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMSO: 20 mg/ml. lambdamax: 334 nm. SMILES: O=C(C=C)N(C/C(C/1=O)=C\C2=CC=C([N+]([O-])=O)C=C2)CC1=C/C3=CC=C([N+]([O-])=O)C=C3. InChi Code: InChI=1S/C22H17N3O6/c1-2-21(26)23-13-17(11-15-3-7-19(8-4-15)24(28)29)22(27)18(14-23)12-16-5-9-20(10-6-16)25(30)31/h2-12H,1,13-14H2/b17-11+,18-12+. InChi Key: GFARQYQBWJLZMW-JYFOCSDGSA-N.