Ac-YVAD-CHO

Ac-YVAD-CHO is an inhibitor of caspase-1/interleukin-1beta converting enzyme (ICE, Ki = 0.76 nM) and an acetylated form of the caspase-1 inhibitor YVAD-CHO (Cay-27420). It is selective for caspase-1 over caspase-4, -5, -8, -9, and -10 (Kis = 163-970 nM), as well as over caspase-2, -3, -6, and -7 (Kis = >10,000 nM for all). It inhibits activation of caspase-1 and IL-1beta in a cell-free assay using LPS-treated THP-1 cell homogenates when used at a concentration of 5 µM. Ac-YVAD-CHO (12.5 µmol/kg) reduces pancreatic levels of IL-18 and serum levels of IL-1beta, as well as reduces pyroptosis of pancreatic cells, in a mouse model of cerulein-induced acute pancreatitis but does not decrease pancreatic expression of NOD-like receptor protein 3 (NLRP3). It increases susceptibility to lethal E. coli infection but prevents LPS-induced death in C3H/HeN mice when administered at doses of 5 and 10 mg/kg, respectively.Formal Name: N-acetyl-L-tyrosyl-L-valyl-N-[(1S)-2-carboxyl-1-formylethyl]-L-alaninamide. CAS Number: 143313-51-3. Synonyms: Caspase-1 Inhibitor I, L 709049, N-Ac-Tyr-Val-Ala-Asp-CHO. Molecular Formula: C23H32N4O8. Formula Weight: 492.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml, PBS (pH 7.2): 5 mg/ml. SMILES: CC(N([H])[C@@H](CC1=CC=C(O)C=C1)C(N([H])[C@@H](C(C)C)C(N([H])[C@@H](C)C(N([H])[C@@H](CC(O)=O)C([H])=O)=O)=O)=O)=O. InChi Code: InChI=1S/C23H32N4O8/c1-12(2)20(23(35)24-13(3)21(33)26-16(11-28)10-19(31)32)27-22(34)18(25-14(4)29)9-15-5-7-17(30)8-6-15/h5-8,11-13,16,18,20,30H,9-10H2,1-4H3,(H,24,35)(H,25,29)(H,26,33)(H,27,34)(H,31,32)/t13-,16-,18-,20-/m0/s1. InChi Key: LPIARALSGDVZEP-SJVNDZIOSA-N.