9-Amino-6-chloro-2-methoxyacridine

9-Amino-6-chloro-2-methoxyacridine (ACMA) is a cell-permeable fluorescent probe that intercalates into DNA. It selectively binds to poly(dA-dT) sequences with the fluorescence lifetime decreasing with incorporation of guanosine. It is used for labeling DNA and displays excitation/emission spectra of 411/475 nm, respectively. ACMA fluorescence is pH-dependent and is quenched when a pH gradient is established, a property that has been utilized in animal- and plant-based studies. It also inhibits acetylcholinesterase with a Ki value of 49 nM.Formal Name: 6-chloro-2-methoxy-9-acridinamine. CAS Number: 3548-09-2. Synonyms: ACMA, NSC 15300. Molecular Formula: C14H11ClN2O. Formula Weight: 258.7. Purity: >98%. Emission: 475 nm. Excitation: 411 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: Methanol: 1 mg/ml, Water: miscible. lambdamax: 218, 277, 340, 412, 435 nm. SMILES: ClC1=CC=C2C(N=C(C=CC(OC)=C3)C3=C2N)=C1. InChi Code: InChI=1S/C14H11ClN2O/c1-18-9-3-5-12-11(7-9)14(16)10-4-2-8(15)6-13(10)17-12/h2-7H,1H3,(H2,16,17). InChi Key: IHHSSHCBRVYGJX-UHFFFAOYSA-N.