7beta,27-dihydroxy Cholesterol

Description: 7beta,27-dihydroxy Cholesterol is an oxysterol and agonist of retinoic acid receptor-related orphan receptor gamma (RORgamma) and RORgammat. [1] It activates RORgamma- or RORgammat-dependent signaling with EC50 values of 691 and 1,045 nM, respectively, in reporter assays using HEK293T cells expressing the recombinant human receptors. 7beta,27-dihydroxy Cholesterol is selective for RORgamma and RORgammat over a panel of eight additional nuclear receptors at 30 µM. It increases IL-17A production in Th17-polarized isolated human na ve CD4+ T cells when used at a concentration of 300 nM. 7beta,27-dihydroxy Cholesterol (60 mg/kg) increases IL-17A production in isolated mouse gammadelta T cells stimulated with 12-myristate 13-acetate and ionomycin . Target: Others. Smiles: C[C@@H](CO)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3[C@@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C. References: Soroosh, P., Wu, J., Xue, X., et al.Oxysterols are agonist ligands of RORgammat and drive Th17 cell differentiationProc. Natl. Acad. Sci. USA111(33)12163-12168(2014)