7-Ethoxyresorufin

7-Ethoxyresorufin (7-ER) is a fluorogenic substrate for, and competitive inhibitor of, the cytochrome P450 (CYP) isoform CYP1A1 (IC50 = 0.1 µM). Upon enzymatic cleavage by CYP1A1, resorufin is released and its fluorescence can be used to quantify CYP1A1 activity. Resorufin displays excitation/emission maxima of 570/580 nm, respectively. 7-ER has been used in the study of arachidonic acid (Cay-90010, Cay-90010.1, Cay-10006607) metabolism and the mechanisms of vasodilation. 7-ER is a noncompetitive inhibitor of neuronal nitric oxide synthase (nNOS, Ki = 0.76 µM).Formal Name: 7-ethoxy-3H-phenoxazin-3-one. CAS Number: 5725-91-7. Synonyms: 7-ER, 7-Ethoxyphenoxazone, Resorufin ethyl ether. Molecular Formula: C14H11NO3. Formula Weight: 241.2. Purity: >95%. Emission: 570 nm. Excitation: 580 nm. Formulation: (Request formulation change), A solid. Solubility: Chloroform: Soluble, DMF: Soluble, DMSO: Soluble. SMILES: O=C1C=CC2=NC(C=CC(OCC)=C3)=C3OC2=C1. InChi Code: InChI=1S/C14H11NO3/c1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h3-8H,2H2,1H3. InChi Key: CRCWUBLTFGOMDD-UHFFFAOYSA-N.