Nonactin, Monactin, and Dinactin Mixture

Nonactin, monactin, and dinactin mixture is a macrotetralide antibiotic. This product is a mixture of the macrotetralide antibiotic homologs nonactin, monactin (Cay-25742), and dinactin (Cay-20752) that all act as ionophores for monovalent cations. Nonactin is a mitochondrial membrane uncoupler and an inhibitor of adenine nucleotide translocase (ANT, IC50s = 4.4, 3.3, 4.7, and 3.3 µM for recombinant human ANT1-4, respectively). It selectively induces apoptosis in cancer cells and induces tumor regression in mouse xenograft models expressing mutant beta-catenin over cancer cells and xenografts expressing wild-type beta-catenin. Monactin induces swelling of rat liver mitochondria in medium containing either potassium or sodium, stimulates respiration, and uncouples oxidative phosphorylation. It also inhibits Wnt signaling via inhibition of TCF-beta-catenin transcriptional activity. Dinactin inhibits T cell proliferation and cytokine production in vitro and reduces pulmonary eosinophilia in antigen-challenged mice.Formal Name: (1R,2R,5R,7R,10S,11S,14S,16S,19R,20R,23R,25R,28S,29S,32S,34S)-2,5,11,14,20,23,29,32-octamethyl-4,13,22,31,37,38,39,40-octaoxapentacyclo[32.2.1.17,10.116,19.125,28]tetracontane-3,12,21,30-tetrone. Molecular Formula: C40H64O12 (for Nonactin). Formula Weight: 736.9. Purity: >95% (mixture of homologues). Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS(pH7.2) (1:2): 0.3 mg/ml, DMSO: 0.25 mg/ml, Ethanol: 1 mg/ml. SMILES: O=C(O[C@@H](C)C[C@@]1([H])O[C@]([C@@H](C)C(O[C@H](C)C[C@]2([H])O[C@@]3([H])CC2)=O)([H])CC1)[C@@H](C)[C@]4([H])CC[C@@](C[C@@H](C)OC([C@H](C)[C@@]5([H])CC[C@](C[C@H](C)OC([C@H]3C)=O)([H])O5)=O)([H])O4. InChi Code: InChI=1S/C40H64O12/c1-21-17-29-9-13-34(49-29)26(6)38(42)46-23(3)19-31-11-15-36(51-31)28(8)40(44)48-24(4)20-32-12-16-35(52-32)27(7)39(43)47-22(2)18-30-10-14-33(50-30)25(5)37(41)45-21/h21-36H,9-20H2,1-8H3/t21-,22+,23+,24-,25-,26+,27+,28-,29-,30+,31+,32. InChi Key: RMIXHJPMNBXMBU-QIIXEHPYSA-N. Origin: Bacterium/Streptomyces griseus.