PDE7A-HEK293 Human Recombinant Cell line, 1x10(6) cells

Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). Format: Each vial contains 1 X 106 cells in 1 ml of 10% DMSO. Functional validation: N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the luciferase reporter whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity. Storage: Store cells in liquid nitrogen upon arrival. Avoid freeze/thaw cycles. References: Malik, R. et al. (2008) Appl. Microbiol. Biotechnol. 77 (5): 1167-1173. Fan Chung, K. (2006). Eur. J. Pharmacol. 533(1-3):110-117.