FcRn: IgG Recycling HMEC-1 Cell Pool

The FcRn: IgG Recycling HMEC-1 Cell Pool is a HMEC-1 cell pool expressing Fc gamma receptor and transporter (FCGRT) (NM_004107.5) and beta-2-microglobulin (B2M) (NM_004048.4), separated by a T2A self-cleaving peptide, under the control of a CMV promoter, introduced into HMEC-1 cells via lentiviral transduction. This cell pool is designed to measure the uptake and recycling of human IgG antibodies (human FcRn does not bind mouse IgG). This cell pool has been validated with Nivolumab, Rituximab, Trastuzumab, anti-PCSK9 and an anti-IL-2Ra antibody. Figure 1: Illustration of FcRn: IgG Recycling HMEC-1 Cell Pool mechanism of IgG FcRn-mediated recycling. Cells are incubated with an antibody (IgG) of interest in a low pH Dilution Buffer, allowing FcRn to bind to IgG. Cells are then washed, and IgG recycling can be analyzed after 24 hours