ADAR1 Activity Luciferase Reporter HEK293 Cell Line

ADAR1 Activity Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor the activity of ADAR1 (adenosine deaminase acting on RNA) enzyme. These cells were engineered to express ADAR1 (NM_001111.5) and an ADAR1 reporter construct comprised of an ADAR1 hairpin target with a stop codon (UAG) susceptible to ADAR1-mediated editing to tryptophan (UUG), located upstream of a firefly luciferase reporter. This cell line has been validated by comparing reporter activation to ADAR1 Responsive Luciferase Reporter HEK293 Cell Line (#82238) and in response to treatment with ADAR1 siRNA. The ADAR1 reporter construct is comprised of an ADAR1 hairpin target with a stop codon (UAG) upstream of the sequence encoding luciferase. In the absence of ADAR1, luciferase is not transcribed, and the cells show no luciferase activity. In the presence of ADAR1 activity, adenine is converted into inosine, encoding now the amino acid tryptophan (UUG) and enabling transcription and expression of luciferase. ADAR1 activity, therefore, directly correlates with luciferase activity.