Hydrogen Peroxide Assay Kit

Cayman's Hydrogen Peroxide Assay Kit provides a simple method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2. In a horseradish peroxidase catalyzed reaction, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin. Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm. Catalase, an H2O2 scavenger, is included in the kit to check specificity of the assay. Reactive oxygen species (ROS), such as H2O2 and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets. This kit provides a valuable method for immunologists to assess the capacity of immune cell killing using ROS.Synonyms: H2O2 Fluorometric Assay Kit. Limit of Detection: 0.02 µM (fluorometric), 0.15 µM (colorimetric). Lower Limit of Quantification: 0.16 µM (fluorometric), 2.5 µM (colorimetric). Origin: Animal/Bovine, Plant/Armoracia rusticana.