One-step TUNEL Flow Cytometry Apoptosis Kit (Red, AF647)

Detection principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry. General Information: Application: DNA Fragmentation. Detection method: FCM. Sample type: Suspension cells, Adherent cells. Assay time: 2.5h-3h. Detection instrument: Flow Cytometry. Dye Type: AF647. Ex/Em (nm): 650/670. Channel set: APC. Other reagents required: PBS buffer (with 1%BSA) (PH7.2 ~ 7.4). Storage: -20°C. Refer to the Manual. Shipping: Ice bag. Expiration date: 12 months.