FLICA(TM) 660 Caspase-1 Assay Kit (25-50 tests)

Kit includes 660-YVAD-FMK red caspase-1 probe, 10x Apoptosis Wash Buffer, Fixative, Technical Protocol. The red FLICA® 660-YVAD-FMK caspase-1 detection probe is comprised of the preferred affinity peptide sequence for active caspase-1 (YVAD), a far-red fluorescent 660 dye label, and a fluoromethyl ketone (FMK) reactive moiety. The resulting probe forms an irreversible, covalent bond with the active caspase-1 enzyme, efficiently labeling the target for detection. Due to its cell permeant nature and fluorescence properties, this probe enables whole cell analysis via common fluorescence detection methods. To use FLICA, add it directly to suspension cell or tissue culture media, incubate, and wash. The cell permeant FLICA 660-YVAD-FMK reagent will efficiently diffuse into cells and irreversibly bind to activated caspase-1 enzymes, thereby retaining the red signal inside caspase-1-positive cells. Cells not bearing active caspase-1 return to a non-fluorescent status after the wash step. The FLICA® 660 Caspase-1 probe has an optimal excitation at 660 nm and optimal emission range from 680-690 nm. As such, it has demonstrated excellent excitation efficiency with a conventional red HeNe laser with a 633 nm excitation, enabling samples to be analyzed with most flow cytometers and fluorescence microscopes equipped with electronic grey scale image capabilities. Can be used for living cell analysis with suspension cell samples, adherent cell samples and thin tissue sections.