VEGF Mouse BioAssay(TM) ELISA Kit

Murine VEGF ELISA Cytokine Development kit contains the key components required for the quantitative measurement of natural and/or recombinant murine VEGF in a sandwich ELISA format. Using the ELISA protocol described below, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay murine VEGF in approximately 1000 ELISA plate wells. Detection Range: 12-1500pg/ml, Crossreactivity: 1. When tested at 50ng/ml the following antigens exhibited 100% crossreactivity: Human VEGF121, VEGF165, Rat VEGF, 2. When tested at 50ng/ml the following antigens exhibited less than 1% crossreactivity: Human PlGF-2 , 3. When tested at 50ng/ml the following antigens did not exhibit significant crossreactivity: Human EGF, EGF Receptor, EG-VEGF, flt3L, GM-CSF, PlGF-1, PlGF-3, SCF, Murine EGF, flt3L, GM-CSF, SCF, Rat SCF, Kit Components: 145984A: Capture Antibody - 1x100ug of antigen-affinity purified rabbit anti-murine VEGF containing 2.5mg D-mannitol., 145984B: Detection Antibody (Biotin) - 1x50ug of biotinylated antigen-affinity purified rabbit anti-murine VEGF containing 2.5mg D-mannitol., 145984C: Murine VEGF Standard - 1x1ug of recombinant murine VEGF containing 2.2mg BSA, 11.0mg D-mannitol., 145984D: Avidin-HRP Conjugate - 1x60ul, Reconstitution and Storage: 145984A: Capture Antibody - Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 100ug/ml., 145984B: Detection Antibody - Centrifuge vial prior to opening. Reconstitute in 0.5ml sterile water for a concentration of 100ug/ml, 145984C: Murine VEGF Standard - Centrifuge vial prior to opening. Reconstitute in 1ml sterile water for a concentration of 1ug/ml., Note:, The reconstituted components are stable for 2 weeks when stored at 2-8°C., Components that have been reconstituted and aliquoted can be stored at -20°C for up to 6 months., 145984D: Avidin-HRP Conjugate - Upon receipt, Avidin-HRP conjugate should be aliquoted into ten 6ul vials and stored at -20°C or lower. Aliquots stored frozen at below -20°C are stable for up to 6 months from date of receipt. Avoid more than one freeze-thaw cycle. Avidin should be used in conjunction with ABTS only. 145859 ELISA Cytokine Development Buffer Kit, BioAssay(TM) Components: 145859A: PBS, 20X,, 145859B: Wash Buffer, 20X,, 145859C: Blocking Buffer, 145859D: Diluent, 20X, 145859E: ABTS Liquid Substrate, 145859F: ELISA Microplates, Storage and Stability: Store kit at -20°C. Stable for 6 months after receipt at -20°C. Aliquot and store reconstituted components at -20°C. Upon receipt, aliquot *145984D into ten 6ul vials. Store at -20°C for up to 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.the cap.