uPA/PLAU BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human uPA/PLAU in cell culture supernates, serum and plasma (heparin, EDTA). Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18kD and 33kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K). , Assay Range:, 62.5pg/ml-4000pg/ml, Sensitivity: < 5 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P00749, Entrez Gene ID: 5328, Kit Components: 96-well plate pre-coated with anti-uPA/PLAU, Human, Lyophilized recombinant uPA/PLAU, Human standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.