Prostate Specific Antigen, Free, Human (PSA, Kallikrein-3, Seminin, Semenogelase, ?-Seminoprotein, K

Prostate Specific Antigen (PSA) is a 33kD single-chain serine proteinase, mainly secreted by the prostate gland (7). PSA serum levels are abnormally elevated in patients with prostate cancer, benign prostatic hypertrophy (BPH) and patients with prostate inflammatory conditions. PSA exists in serum in at least 3 different forms: Free PSA, alpha-2 macroglobulin bounded PSA and alpha-1-anti-chymotrypsin bounded PSA (5). In combination with the digital rectal exam and/or transrectal ultrasound, PSA level has been used as a Standard marker for early prostate cancer screening program. Free PSA is the unbound form of prostate specific antigen (PSA). Studies (5, 6) have suggested that the percentage of Free PSA in total PSA is lower in patients with prostate cancer than those with benign prostate hyperplasia. The Free to total PSA ratio is now being introduced and studied as an additional tool to help clinician to decide if a patient needs more aggressive evaluation, such as prostate biopsy, to check for prostate cancer. Kit Components: 1. Free PSA Microtiter Plate, 1x96 wells, 2. Ab (HRP), 1x12ml, 3. Free PSA Standard-10ng/ml, 1x1 vial , 4. Free PSA Standard- 5ng/ml, 1x1 vial, 5. Free PSA Standard-2ng/ml, 1x1 vial, 6. Free PSA Standard-1ng/ml, 1x1 vial, 7. Free PSA Standard - 0.5ng/ml, 1x1 vial, 8. Free PSA Standard - 0.1ng/ml, 1x1 vial, 9. Free PSA Standard - 0ng/ml, 1x1 vial, 10. Substrate A, 1x10ml, 11. Substrate B, 1x10ml, 12. Stop Solution, 1x10ml, 13. Sample Diluent, 1x10ml, Storage and Stability: Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.