Naveni PD1/PD-L1 Atto647N

The Naveni PD1/PD-L1 is an in situ proximity ligation assay kit for the detection of the interaction of PD1 and PD-L1. This in situ kit gives a high signal-to-noise ratio and has an out-standing specificity. The kit is comatible with immunofluorescence co-staining for visualization of the tumor microenvironment. The kit has been validated on FFPE human tissue samples and tested in fresh frozen tissue. Not recommend for mouse tissue. For research use only. Not for use in diagnostic procedures. The kit is based on our NaveniTM Proximity Ligation Technology, with two Navenibodies conjugated to proprietary oligo arms. The technology ensures specific and sensitive detection. One Navenibody binds the PD1 protein, and the other Navenibody binds PD-L1 . Probes in close proximity will give a strong and distinct signal, creating a specific and sensitive detection method for the interaction between PD1 and PD-L1. Protein function: Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. [The UniProt Consortium] Included in the kit: Navenibody targeting human PD1 protein based on clone EH33 CST Navenibody targeting human PD-L1 protein based on clone SP142 Abcam RabMAb(R) Detection fluorophore Atto647N Buffers for blocking and dilutions and all detection reagents The Navenibody solution has a working volume of 4000µl (sufficient for approx. 100 reactions) Not included in the kit: Tris EDTA antigen retrieval buffer pH 9 Wash buffer DAPI nuclear stain Mounting medium