KLK1 (Kallikrein-1) BioAssay(TM) ELISA Kit (Mouse)

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262aa which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse KLK1/Kallikrein 1. Sensitivity: <12pg/ml, Range: 62.5pg/ml-4000pg/ml, Specificity: Recognizes natural and recombinant Mouse KLK1/Kallikrein 1. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse KLK1/Kallikrein 1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from rat specific for KLK1/Kallikrein 1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: I25-D261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK1/Kallikrein 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse KLK1/Kallikrein 1 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Klk1 antibody, 1x plate, Lyophilized recombinant Mouse Klk1 standard, 2x10ng/tube, Biotinylated anti-Mouse Klk1 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.