Human 12-LO (Arachidonate 12-Lipoxygenase) CLIA Kit

ELISA Type: CLIA. Sensitivity: 37.5 pg/mL. Range: 62.5--4000 pg/mL. Protein function: Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species (PubMed:17493578, PubMed:1851637, PubMed:8319693, PubMed:8500694, PubMed:18311922). Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid (PubMed:8250832, PubMed:17493578, PubMed:22984144, PubMed:24282679, PubMed:8319693, PubMed:8500694). In contrast does not react towards methyl esters of linoleic and arachidonic acids. Also catalyzes the epoxidation of double bonds of polyunsaturated fatty acids such as 14S-hydroperoxy- docosahexaenoate (DHA) resulting in the formation of (13S,14S)-epoxy- DHA (PubMed:23504711). Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4 (PubMed:8250832). Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation (PubMed:8319693, PubMed:8500694). It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor (PubMed:9751607, PubMed:16638750). Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance (PubMed:23578768). May also play a role in the control of cell migration and proliferation (PubMed:22237009). [The UniProt Consortium]