USP10 Inhibitor Screening Assay Kit

Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
BPS-79094 96 rxns - -

3 - 15 Werktage*

1.245,00 €
 
The USP10 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity... mehr
Produktinformationen "USP10 Inhibitor Screening Assay Kit"
The USP10 Inhibitor Screening Assay Kit is a fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme USP10 (Ubiquitin Specific Peptidase 10) for screening and profiling applications. The kit comes in a convenient 96-well format and contains enough purified recombinant human USP10 protein, Ubiquitinated-AMC substrate, and assay buffer for 100 reactions. To determine the effect of an inhibitor on USP10 activity the enzyme should be preincubated with or without the test inhibitor prior to adding the Ub-AMC substrate to the reaction. The assay was functionally validated using Ub-Aldehyde, a potent inhibitor of DUB subfamilies Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU) and Machado-Josephin Domain (MJD) proteases. Ubiquitin-AMC (Ub-AMC) is a fluorogenic substrate for ubiquitin hydrolases, based on the C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). In the conjugated form the energy emitted from the fluorochrome AMC is quenched. Upon release from Ub, AMC emissions are no longer quenched and fluorescence with lambdaexcitation/lambdaemission maxima of 350/460 nm is emitted. The increase in fluorescence is proportional to the DUB activity.
Schlagworte: Ubiquitin thioesterase 10, Deubiquitinating enzyme 10, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10,
Hersteller: BPS Bioscience
Hersteller-Nr: 79094

Eigenschaften

Anwendung: Enzyme kinetics, small molecule inhibitor screening, drug discovery, HTS
Spezies-Reaktivität: human

Handhabung & Sicherheit

Lagerung: -80°C
Versand: -80°C (International: °C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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