Trypsin-2 Activity Assay Kit

The Trypsin-2 Activity Assay Kit is a colorimetric assay kit designed to measure trypsin-2 protease activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant trypsin-2, substrate, and assay buffer for 100 enzyme reactions. Figure 1: Illustration of the assay principle. Upon proteolysis, trypsin-2 cleaves the chromogenic substrate at the C-terminal end releasing p-nitroanilid (pNA), which produces a yellow color that is measurable photometrically at lambda=405 nm. The increase in color is proportional to trypsin-2 activity.