Thioredoxin Fluorometric Activity Assay Kit

Cayman's Thioredoxin Fluorescent Activity Assay Kit provides a convenient method of detecting Trx activity in cell lysate. Measurement of the Trx activity is based on the reduction of eosin-labeled insulin disulfides by Trx. The reduction of insulin disulfides results in a release of eosin and an increase in fluorescence that can be detected at 560 nm. Trx becomes oxidized during this process, and oxidized Trx is recycled back to the reduced state by Trx reductase. Under circumstances in which the Trx activity rate is limiting, the rate of fluorescence increase is directly proportional to the Trx activity in the sample.Synonyms: Trx, Txn. Emission: 560 nm. Excitation: 520 nm. Origin: Animal/Bovine.