Succinate Dehydrogenase (SDH) Activity Assay Kit

Detection instrument: Microplate reader. Detection principle: SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm. Sample type: animal tissue, cell. Research area: Enzymes,Tricarboxylic Acid(TCA) Cycle. Assay time: 10 min. Sensitivity: 0.83 U/L. Detection range: 0.83-65.42 U/L. Recovery rate: 105. Inter CV: 4. Intra CV: 2