ROS Detection Cell-Based Assay Kit (DCFDA)

2,7-Dichlorofluoroscin Diacetate (DCFDA or 2,7-dichlorodihydrofluoroscein diacetate) is a widely-used, cell-permeable redox sensitive fluorescent probe that is oxidized by ROS and certain RNS to yield the highly fluorescent product 2,7-dichlorofluoroscein. Cayman's ROS Detection Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control.