Pyruvate Carboxylase (PC) Activity Assay Kit

Detection principle: The product of the PC-catalyzed substrate reacts with the reducing agent. The reducing agent has a maximum absorbance at 340 nm, and the enzyme activity of PC is calculated by calculating the amount of reducing agent consumed in the reaction. Performance characteristics: Sample type: serum,plasma,animal tissue,cell. Sensitivity: 6.43 U/L. Detection range: 6.43-876.53 U/L. Detection method: Colorimetric method. Assay type: Enzyme Activity. Assay time: 35 min. Precision: Average inter-assay CV: 3.9-9.7%. Average intra-assay CV: 0.6-1.4%. Other reagents required: Double distilled water, Normal saline (0.9% NaCl). Storage: -20? .Valid period: 12 months. Protein function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. [The UniProt Consortium]