Peroxidase (POD) Activity Assay Kit (Plant samples)

Detection principle Plant peroxidase, a member of the superfamily of peroxidase, catalyzes the redox reaction between H2O2 and various reductants. The plant peroxidase has the same general structure and consists of iron porphyrin IX and ten alpha-helixes. Based on the difference of primary structure, the superfamily of plant peroxidase can be divided into three types: class I (intracellular type), class II (extracellular type of fungi) and class III (secreted type of plant). Performance characteristics Synonyms POD Sample type Plant tissue Sensitivity 0.01 U/mL Detection range 0.01-100 U/mL Detection method Colorimetric method Assay type Enzyme Activity Assay time 60 min Precision Average inter-assay CV: 5%Average intra-assay CV: 3.2% Other instruments required Spectrophotometer (240 nm), Micropipettor, Vortex mixer, Centrifuge, 37 °C Incubator Other reagents required PBS (0.01 M, pH 7.4) Storage 2-8 °C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-100 U/mL). The recommended dilution factor for different samples is as follows (for reference only): Sample type Dilution factor 10% Green pepper tissue homogenate 1 10% Chive leaf tissue homogenate 1 10% Photinia leaf tissue homogenate 1 10% Epipremnum aureum tissue homogenate 1 10% Mushrooms tissue homogenate 1 10% White radish tissue homogenate 1 Note: The diluent is PBS (0.01 M, pH 7.4).