PDE4D2 assay kit

The PDE4D Assay Kit is designed for identification of inhibitors of PDE4D2 using fluorescence polarization. Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has been associated with asthma pathophysiology and bone formation. The PDE4D gene encodes at least 9 different isoforms. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4D to the binding agent. The PDE4D inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE4D2 enzyme, fluorescently labeled PDE4D substrate (cAMP), binding M361agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4D Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4D for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.