NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit

Detection principle: Isocitrate dehydrogenase converts isocitrate to alpha- ketoglutaric acid, while converting NADP+ to NADPH. Under the action of electron coupling agents, the generated NADPH has a characteristic absorption peak at 450 nm. The activity of NADP-IDH can be calculated by measuring the change of absorbance value at 450 nm. Performance characteristics: Sample type: tissue,cell. Sensitivity: 0.10 U/L. Detection range: 0.10-50.47 U/L. Detection method: Colorimetric method Assay. type: Enzyme Activity. Assay time: 70 min. Precision: Average inter-assay CV: 4.3-11.4%. Average intra-assay CV: 5.0-6.0%. Other reagents required: Double distilled water. Storage: -20?. Valid period: 12 months. Protein function: Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed:10521434, PubMed:19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed:10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency. [The UniProt Consortium]