Live or Dead(TM) Yeast Viability Assay Kit

The Live or Dead(TM) Yeast Viability Assay Kit is a reliable and robust assay that allows researchers to quantitatively distinguish live and dead yeast within minutes using flow cytometry. This kit contains two ready-to-use nucleic acid stains: MycoLight(TM) Green JJ98, a replacement for SYTO(TM) 9, and propidium iodide. MycoLight(TM) Green JJ98 is a green fluorescent nucleic acid stain that labels all yeast populations - live yeast with intact membranes and dead yeast with damaged membranes. In contrast, propidium iodide only stains dead yeast with damaged membranes. In dead yeasts where both dyes are present, the fluorescence of MycoLight(TM) Green JJ98 is notably reduced due to FRET by propidium iodide. As a result, live yeast with intact membranes will stain fluorescent green, and dead yeast with damaged membranes will stain fluorescent red.