KRAS(G12D) Nucleotide Exchange Assay Kit

The KRAS(G12D) Nucleotide Exchange Assay is a homogeneous assay designed for the screening and profiling of KRAS(G12D) antagonists/inhibitors by using BODIPY-GDP to monitor the GDP or GTP binding status. The kit can be used with two different protocols for greater flexibility, either titrating the inhibitor at a fixed GTP concentration or titrating the GTP at a fixed inhibitor concentration. BODIPY(R) FL GDP, is a mixed isomer in which the BODIPY(R) FL fluorophore has been attached to the 2' or 3' position of the ribose ring via a linker. BODIPY(R) FL dye is a green-fluorescent dye with similar excitation and emission to fluorescein or Alexa Fluor(TM) 488. It is characterized by a high extinction coefficient and high quantum yield and is relatively insensitive to pH changes. The dye has an excited-state lifetime of 5 nanoseconds or longer, ideal for fluorescence polarization-based assays.