JMJD3 chemiluminescent assay kit

The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3 is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3. The JMJD3 Assay Kit comes in a convenient format, with 8-well strips precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD3 for 100 enzyme reactions. The key to the JMJD3 Direct Activity Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but non-specific demethylase activity may be detected. Components: JMJD3-4 µg, primary antibody 6-25 µl, secondary HRP-labeled antibody 2- 10 µL, 4x JMJD3 assay buffer 3 ml, blocking buffer 50 ml, HRP chemiluminescent substrate 6 ml ea (2 components), 12 x 8-well strips