HDAC Fluorogenic Assay Kit (Green)

The Fluorogenic HDAC Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) class 1 activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g. HeLa nuclear extract or purified HDAC enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader at 485 nm (excitation) and 528 nm (emission). COMPONENTS : Fluorogenic HDAC substrate2, 50 ul at 5 mM, 2 x HDAC developer, 6 ml, HDAC assay buffer, 10 ml, Trichostatin A, 100 ul at 200 uM, One black plate, HDAC2 (5 ug) for the positive control. Applications : Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.