EZH2 Homogenous Assay Kit

The EZH2 Homogenous Assay Kit is designed to measure activity of the EZH2 using purified enzyme complex or cell extracts containing EZH2. EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27. The EZH2 Homogenous Assay Kit comes in a convenient AlphaScreen or AlphaLISA format, with a 384-well plate, histone substrate mixture (his-tagged), primary antibody, methylation assay buffer, and purified EZH2 for 384 enzyme reactions. The key to the EZH2 Homogenous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.