DNA Polymerase theta Assay Kit

The DNA Polymerase theta Assay Kit is a fluorogenic assay designed to measure DNA Polymerase theta activity for screening and profiling applications, utilizing the fluorescent nucleic acid dye GroovyGreen(TM) for the quantitation of double-stranded DNA (dsDNA) products. The kit comes in a convenient 96-well format and contains enough recombinant DNA Polymerase theta enzyme (amino acids 1792-2590), DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions.  DNA Polymerase theta uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecules (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can be quantified by the addition of a fluorescent dsDNA-binding dye which distinguishes dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.