Cell Meter(TM) Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*

Ex: 576 nm. Em: 598 nm. The detection of intracellular hydroxyl radical is of central importance to understanding proper cellular redox regulation and the impact of its dysregulation on various pathologies. The hydroxyl radical (HO·) is one of the reactive oxygen species (ROS) highly reactive with other molecules to achieve stability. In general, hydroxyl radical is considered to be a harmful by-product of oxidative metabolism, which can cause molecular damage in living system. It shows an average lifetime of 10-9 nano seconds and can react with nearly every biomolecule such as nuclear DNA, mitochondrial DNA, proteins and membrane lipids. AAT Bioquest's Cell Meter(TM) Mitochondrial Hydroxyl Radical Detection Kit is optimized for detecting hydroxyl radical in mitochondria. MitoROS(TM) OH580 is live-cell permeant probe and can rapidly and selectively target hydroxyl radical in live cells. It generates red fluorescence when it reacts with OH·, and can be easily read at Ex/Em= 540/590 nm. Cell Meter(TM) Mitochondrial Hydroxyl Radical Detection Kit provides a sensitive fluorimetric probe to detect OH· in live cells with one hour incubation. This kit can be used for fluorescence microplate readers and fluorescence microscopy applications.