Activin A: Activin RIIB[Biotinylated] Inhibitor Screening Assay Kit

The Activin A: Activin RIIB [Biotinylated] Inhibitor Screening Assay Kit is an ELISA-based assay designed to measure the binding between Activin A and Activin RIIB (receptor IIB), also known as ACVR2B, protein for screening and profiling applications. This kit comes with enough purified Activin A (amino acids 311-426) and biotinylated Activin Receptor IIB (amino acids 19-137), streptavidin-HRP, assay buffer, and detection reagent for 100 enzyme reactions. A 96-well plate is coated with Activin A protein. After coating and blocking, biotinylated Activin RIIB, also known as ACVR2B, is added in an optimized assay buffer. Next, unbound biotinylated Activin RIIB is washed away, and the plate is incubated with streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of Activin RIIB binding to Activin A.