Anti-phospho-RPA2, (T21) (Replication Protein A2)

RPA2 is a 32kD DNA-binding protein that constitutess one of three subunits comprising the PRA heterotrimer complex. In conjunction with 70kD RPA1 and 14kD RPA3, RPA2 participates in DNA replication, recombination and repair. Human RPA2 is 270aa in length. It contains a Gly/Ser-rich N-terminus (aa1-33), a DNA-binding domain (aa43-171) and a protein-interaction C-terminus (aa187-270). Phosphorylation of the N-terminus on Ser4/8/23/29/33, plus Thr21, regulates RPA complex interactions with DNA repair and replication complexes. There are multiple splice variants. Three contain N-terminal extensions: one shows an 88aa insertion after Ser4, another shows a 12aa substitution for aa1-4, and a third shows a four aa insertion after Ser4. There is also a deletion of aa93-98, and a potential truncation after Gln175. Over aa141-270, human RPA2 shares 83% aa identity with mouse RPA2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.