Anti-phospho-p53 (Ser392)

Anti-p53 pS392 antibody is directed against p53. The anibody was affinity purified from monospecific antiserum by immunoaffinity purification and is specific for the phosphorylated protein. The immunolabeling is completely eliminated by ?-phosphatase treatment. Reactivity is expected the following species based on 100% sequence homology: human and rat. Cross reactivity with p53 from other species has not been determined Protein function: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Induces the transcription of long intergenic non- coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53- inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. [The UniProt Consortium]